Recombinant WNV NS3 protease and NS2b Fusion Protein, His tagged

• Cat.No.: NS3 Protease-19W
• Product Overview: His-tagged recombinant WNV NS3 protease (residues 1 to 184) was expressed as a fusion protein with the cofactor NS2b (residues 49 to 96) and a linker sequence (GGGGSGGGG) in E. coli. Its activity can be measured by FRET peptides

Specification

• Species: WNV
• Source: E.coli
• Tag: His
• Protein Length: NS3 protease: 1-184aa; NS2b: 49-96
• Description: West Nile virus (WNV) is a member of the flavivirus genus, which contains many significant human pathogens including Dengue virus (Den), Japanese Encephalitits virus (JE), and Yellow Fever virus (YF). WNV is a small, enveloped virus with a single stranded, positive sense 11kb RNA genome, which encodes a polyprotein precursor. This polyprotein must be cleaved co- and post-translationally to produce ten functional proteins: three structural (C, prM and E) and 7 nonstructural (NS1, NS2A, NS2B, NS3, NS4A,NS4B and NS5). WNV NS3 protease is absolutely essential (along with viral-encoded cofactor NS2B) for post-translational cleavage and viral replication. As a result, this protease is a potential therapeutic target.
• Tag: His
• Form: Solution
• Molecular Mass: 32 kDa
• Purity: ≥95% by SDS-PAGE
• Applications: FRET; Protease activity
• Usage: Research use
• Unit Definition: One unit of protease hydrolyzes 1 picomole of Pyr-RTKR-AMC substrate from West Nile Virus Protease Assay Kits per minute at pH 8.0 at 30 centigrade.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 100 μg/ml
• Storage Buffer: 25 mM Tris-HCl, pH 7.5, 150 mM NaCl
• References: 1. Brinton, MA. Annu Rev Microbiol. 56, 371 (2002)
  2. Tyndall, JD. et al. Chem. Rev. 105, 973 (2005).
  3. Loughlin, WA. et al. Chem. Rev. 104, 6085 (2004)
  4. D'Arcy, A.et al. Acta Cryst. F62, 157 (2006).

Gene Information

• Official Symbol: NS3 protease; NS2b
• Synonyms: NS3 protease; NS2b; Genome polyprotein; GP1; WNV NS3 protease; WNV NS2b; West Nile Virus; WNV
• UniProt ID: Q9Q6P4