Recombinant Yersinia pseudotuberculosis yopH Protein (163-468, Ready-to-Use)

• Cat.No.: yopH-21Y
• Product Overview: The PTP catalytic domain (a.a. 163 to a.a. 468) of Yersinia enterocolitica YopH is expressed in E. coli. The tyrosine-protein phosphatase YopH selectively hydrolyzes only phosphortyrosine-containing proteins and contains a Cys residue essential for catalysis.

Specification

• Description: Essential virulence determinant. This protein is a protein tyrosine phosphatase. The essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.
• Source: E. coli
• Species: Yersinia pseudotuberculosis
• Molecular Mass: 59 kDa
• Protein Length: 163-468
• Purity: > 90% by SDS-PAGE
• Usage: Remove phosphate group from phosphorated proteins, Phosphatase assay, ELISA, WB, SDS-PAGE, Immunogen, etc
• Storage: Stable for 12 months at -80 centigrade, Avoid freeze/thaw cycles
• Storage Buffer: 50mM Tris-HCl, 200mM NaCl, 10% Glycerol, pH 8.0

Gene Information

• Gene Name: yopH T3SS effector protein-tyrosine-phosphatase YopH [ Yersinia pseudotuberculosis IP 32953 ]
• Official Symbol: yopH
• Synonyms: yopH; T3SS effector protein-tyrosine-phosphatase YopH; EC 3.1.3.48; yop2b
• Gene ID: 66841085
• Protein Refseq: WP_002213278
• UniProt ID: P08538