Recombinant YFV Envelope Protein, 287-679, C-sheep Fc tagged

• Cat.No.: E-04Y
• Product Overview: Recombinant Yellow Fever virus Envelope protein (NCBI accession number AWB15005AA287-679) produced in insect cells. Protein contains a C-terminal sheep Fc-tag. The protein waspurified from culture supernatant by Protein G affinity and ion exchange chromatography.

Specification

• Description: Translation of the positive-stranded viral genomic RNA in infected cells results in the synthesis of a polyprotein precursor of 3411 amino acids, which when proteolytically processed, generates 10 viral proteins. These are (from the amino- to carboxi-terminus of the precursor polyprotein): C; prM/M; E; NS1; NS2A; NS2B; NS3; NS4A; NS4B and NS5. The first three are viral structural proteins that form the virus particle together with the RNA molecule, namely capsid (C, 12–14kDa), membrane (M of 8 kDa, its precursor prM of 18–22 kDa), and envelope (E, 52–54 kDa). These are encoded in the first quarter of the genome. The remainder of the genome encodes the non-structural proteins (NS), numbered from 1 to 5 (NS1 to NS5) in accordance with the order of synthesis. E protein is required for receptor binding and viral attachment, fusion, penetration, hemagglutination, host range and cell tropism. It also has an important role in immunological anti-virus response, eliciting neutralizing antibodies and inducing protective response. Native E protein forms homodimers and it undergoes conformational rearrangement into a fusogenic homotrimer after entering cells by receptor-mediated endocytosis. This conformational change occurs in the lower pH environment of the endosome where viral lipid envelope fusion with endosomal membrane, releases the nucleocapsids into the cells cytoplasm. Each E protein monomer has a molecular mass of 50-55 kDa and has three distinct domains: domain I, II and III. Domain III is the immunoglobulin-like receptor binding domain and is recognized by virus-neutralizing antibodies, making it a target for diagnostic assays. Yellow fever continues to be a public health concern inmany countries of Africa and the Americas. It is estimated that 200000 cases and 30000 deaths are attributable to yellow fever annually.
• Source: Insect cells
• Species: YFV
• Tag: C-sheep Fc
• Protein length: 287-679
• Form: liquid
• Storage: Short Term Storage: -80 centigrade
  Long Term Storage: -80 centigrade
• Concentration: 0.75 mg/ml
• Storage Buffer: Dulbecco's phosphate buffered saline (DPBS) pH 7.4.

Gene Information

• Gene Name: POLY polyprotein [ Yellow fever virus ]
• Official Symbol: POLY
• Synonyms: POLY; polyprotein; polyprotein; core protein C; anchored core protein C; M protein precursor; matrix protein M; envelope protein; non-structural protein NS1; non-structural protein NS2a; non-structural protein NS2b; non-structural protein NS3; non-structural protein NS4a; 2K protein; non-structural protein NS4b; RNA-dependent RNA polymerase; Envelope; E; env
• Gene ID: 1502173
• Protein Refseq: NP_041726
• UniProt ID: P03314