Recombinant Zebrafish EFNB2A protein(Met 1-Ala222), His-tagged
Cat.No. : | EFNB2A-1142Z |
Product Overview : | Recombinant Zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed in HEK293 with a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Zebrafish |
Tag : | C-His |
Protein length : | Met 1-Ala222 |
Form : | Lyophilized from sterile PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml. |
Molecular Mass : | The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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