Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?
02/01/2023
1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities.
2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability.
3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization.
4.Wash the plate with a suitable buffer to remove any unbound protein.
5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein.
6.Wash the plate again to remove any unbound blocking agent.
7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.
