S-Glutathionylated Protein Detection Kit
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|Product Overview :||S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.|
|Storage :||This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box. Once the kit has been opened, store the vials of PSSG Assay Buffer (10X) and PSSG Lysis Buffer at room temperature. Store the remaining components at -20°C.|