"SETD7/9" Related Products

SET7/9 Methyltransferase Inhibitor Screening Assay Kit

Cat.No.: Kit-2021
Description: SET7/9 is an MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. In the SET7/9 MT Inhibitor Screening Assay the transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates SAH, which is rapidly converted to urate and H2O2 using an enzyme mixture provided in the kit. A subsequent reaction between H2O2 and ADHP produces the highly fluorescent compound resorufin.
Storage: -80°C
Kit Components: MT Assay Buffer: 1 vial/20 ml; -20°CMT Assay Buffer Additive: 1 vial/0.2 ml; -20°C MT Enzyme Mixture: 3 vials/0.3 ml; -80°CMT Fluorometric Mixture: 3 vials; -20°CMT Assay S-Adenosylmethionine: 3 vials; -80°CSET7/9 (human recombinant): 2 vials/0.1 ml; -80°CDMSO Assay Reagent: 1 vial/1 ml; Room temperature HCl Assay Reagent (20 mM): 1 vial/1 ml; -20°CSET7/9 Acceptor Peptide: 2 vials/0.6 ml; -20°CMT Assay AdoHcy Positive Control: 1 vial/200 µl; -80°CHalf Volume 96-Well Solid Plate (black): 1 plate; Room temperature 96-Well Cover Sheet: 1 cover; Room temperature
Products Types

◆ Assay kits

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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