Synthetic Human Glucagon

• Cat.No.: GCG-370H
• Product Overview: Synthetic HumanGlucagon is a single, non-glycosylated, polypeptide chain containing 29 aminoacids and having a MW 3.483 kDa.

Specification

• Species: Human
• Source: Synthetic
• Tag: Non
• Description: The protein encodedby this gene is actually a preproprotein that is cleaved into four distinctmature peptides. One of these, GCG, is a pancreatic hormone that counteractsthe glucose-lowering action of insulin by stimulating glycogenolysis andgluconeogenesis. GCG is a ligand for a specific G-protein linked receptorwhose signaling pathway controls cell proliferation. Two of the otherpeptides are secreted from gut endocrine cells and promote nutrientabsorption through distinct mechanisms. Finally, the fourth peptide issimilar to glicentin, an active enter GCG.
• Form: Lyophilized with noadditives.
• Purity: Greater than 98.0%as determined by analysis by RP-HPLC and SDS-PAGE gel.
• Amino Acid Sequence: H-His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-OH
• Reconstitution: Centrifugevial prior to opening. First add sterile water to the vial to fullysolubilize the protein to a concentration not less than 100 ug/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
• EndotoxinLevel: Less than0.1 ng/ug (1 EU/ug) of the protein.
• Storage: Lyophilizedsamples are stable at room temperature for 3 weeks, but it is recommended tostore them desiccated at -20°C to -80°C. Upon reconstitution, protein shouldbe stored at 2-4°C for one week and for six months at -20°C to -80°C. Add acarrier protein (0.1% HSA or BSA) as a stabilizer for long term storage.Avoid repeated freeze-thaw cycles.
• OfficialSymbol: GCG

Gene Information

• Gene Name: GCGglucagon [ Homo sapiens ]
• Synonyms: GCG; glucagon;GLP1; GLP2; GRPP; OTTHUMP00000204570; glucagon-like peptide 1; glucagon-likepeptide 2; glicentin-related polypeptide; Glicentin; Oxyntomodulin
• Gene ID: 2641
• mRNA Refseq: NM_002054
• Protein Refseq: NP_002045
• MIM: 138030
• UniProt ID: P01275
• Chromosome Location: 2q36-q37
• Pathway: Class B/2 (Secreting family receptors); Diabetespathways; FOXA1 transcription factor network; G alpha (q) signaling events; GPCRdownstream signaling; Glucagon signaling in metabolic regulation; Glucagon-typeligand receptors; Incretion Synthesis, Secretion, and Inactivation; Integrationof energy metabolism; Metabolism; Regulation of Insulin Secretion; SignalTransduction; Signaling by GPCR; Synthesis, Secretion, and Deacylation ofGhrelin; Synthesis, Secretion, and Inactivation of Glucagon-like Peptide-1(GLP-1)
• Function: hormone activity; receptorbinding;glucagon receptor binding

Citation

Decarboxylated osteocalcin, a possible drug for type 2 diabetes, triggers glucose uptake in MG63 cells

Journal: World Journal of Diabetes    PubMed ID: 34326958    Data: 2021/7/15

Authors: Shi Jin, Xiao-Cen Chang, Jian Du

Article Snippet:MG63, HEK-293, and Jurkat cells were purchased from American Type Culture Collection.MG63, HEK-293, and Jurkat cells were purchased from American Type Culture Collection.. Synthetic human dcOC was purchased from Creative BioMart (United States) .. Antibodies specific for p-AKT (Ser473), AKT, HIF-1α, GLUT1, and Runx2 were purchased from Cell Signaling Technology.Antibodies specific for p-AKT (Ser473), AKT, HIF-1α, GLUT1, and Runx2 were purchased from Cell Signaling Technology.