"Histone" Related Products

    Tri-Methyl Histone H3-K36 Quantification Kit (Colorimetric)

    Cat.No. : Kit-0820
    Product Overview : Tri-Methyl Histone H3-K36 Quantification Kit (Colorimetric) is use for measuring tri-methylation of histone H3-K36.
    Description : Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. SET2 is a histone methyltransferase that catalyzes methylation of histone H3 at lysine 36 (H3-K36) in mammalian cells.H3-K36 tri-methylation is associated with transcriptionally active genes. Increased H3-K36 methylation is also found to be linked to the Sotos syndrome and leukemia-associated protein NSD1 and the Huntington"s disease protein HYPB. The H3-K36 tri-methylation can be changed by inhibiton or activation of HMTs.Thus quantitative detection of tri-methyl histone H3-K4 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HMT-targeted drugs. The Tri-Methyl Histone H3-K36 Quantification Kit (Colorimetric) provides a tool for measuring tri-methylation of histone H3-K36.
    Applications : For specifically measuring histone H3-K36 trimethylation using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
    Usage : For research use only (RUO)
    Storage : Upon receipt, store C3 and Standard control at –20°C. Store all other components at 4°C away from light. The components of the kit should be stable for 6 months when stored properly.Note: Check if buffers C1 and C2 contain salt precipitates before using. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are redissolved.
    Kit Components : C1 (10X wash buffer) 20 mlC2 (antibody buffer) 12 mlC3 (detection antibody, 1 mg/ml)* 10 µlC4 (color developer) 10 µlC5 (stop solution) 6 mlStandard control (100 µg/ml)* 20 µl8 well sample strips (with frame) 98 well standard control strips* 3* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
    Features & Benefits : Quick and efficient procedure, which can be finished within 2.5 hours.Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.Specifically capturing tri-methylated H3-K36 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.The control is conveniently included for quantification of the amount of tri-methylated H3-K36.Strip microplate format makes the assay flexible: manual or high throughput.Simple, reliable, and consistent assay conditions.

    Online Inquiry

    Note: There will be extra charge for optional service!

    Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

    Optional requirements on this protein    +Expand

    Price Inquiry

    Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.