TrkB Functional Assay Kit

• Cat.No.: Kit-1629
• Product Overview: RTK Functional cell lines are engineered to co-a ProLink (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an untagged co-receptor. Activation of the RTK-PK induces receptor dimerization, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Specification

• Tag: Non
• Size: 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
• Target Species: Human
• Storage: Short term (2 weeks): Store in vapor phase of liquid N2
• Kit Components: 1.25 x 10^6 cells per vial in 0.1 mL (kit contains cells, detection reagents and plate(s))