Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

• Cat.No.: Kit-0845
• Product Overview: Simple procedure; takes ~ less than 40 minutes
  Fast and convenient
  Sensitive assays for measuring Xanthine Oxidase activity in various biological samples

Specification

• Tag: Non
• Description: Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. We have developed an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with OxiRed Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
• Applications: The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
• Usage: For Research Use Only! Not For Use in Humans
• Storage: -20°C
• Size: 100 assays
• Kit Components: • XO Assay Buffer
  • OxiRed Probe (in DMSO)
  • XO Enzyme Mix
  • XO Substrate Mix
  • XO Positive Control
  • H₂O₂ Standard (0.88 M)
• Target Species: Mammalian
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Compatible Sample Types: Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc.