Acitve Recombinant Mouse IL12A & IL12B protein(Met1-Ala215 & Met1-Ser335), His&hFc-tagged

• Cat.No.: IL12A & IL12B-1385M
• Product Overview: Recombinant Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; RecombinantMouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified and expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Ala215 & Met1-Ser335
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein. The EC50 of Mouse IL12A & IL12B Heterodimer Protein is 50.2-117.2 ng/ml. 2. Measured by its ability to induce Interferon gamma secretion by Human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 1-5ng/mL.
• Molecular Mass: The recombinant Mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus ]
• Official Symbol: IL12A; IL12B
• Synonyms: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896