Active Glycosylated Recombinant Human CSF1 Homodimer

• Cat.No.: CSF1-045H
• Product Overview: Recombinant human macrophage colony-stimulating factor (M-CSF) is a homodimer composed of two glycosylated polypeptide chains, each containing 149 amino acid residues.

Specification

• Species: Human
• Source: Yeast
• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.
• Form: Sterile lyophilized powder
• Molecular Mass: 17.4kDa (Monomer)
• Bio-activity: The specific activity is determined by a cell-proliferation assay using the M-NFS-60-dependent cell line and is ≥1.5 × 10^8 IU/mg.
• Endotoxin: <1 EU/μg determined by LAL.
• Purity: ≥97%
• Storage: Lyophilized samples remain stable for 36 months when stored at –20 to –70 centigrade from the date of manufacture. After reconstitution, the cytokine can be kept for one month at 2–8 centigrade under sterile conditions, or for three months at –20 to –70 centigrade in a manual-defrost freezer, without significant loss of activity. Avoid repeated freeze-thaw cycles.
• Storage Buffer: This product is supplied as a sterile lyophilized powder in a buffer of PBS, pH7.4.
• Reconstitution: Before opening the vial, centrifuge at 10, 000 rpm for 30–60 seconds, or at 3, 000 rpm for 5 minutes, then reconstitute with 0.2 mL of sterile water.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens (human) ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603