Active GMP Recombinant Human BMP2 Protein

• Cat.No.: BMP2-119HG
• Product Overview: Recombinant Human BMP2, Gln283-Arg396, was produced in CHO cell under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 283-396 a.a.
• Form: Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.
  The specific activity of recombinant human BMP-2 is approximately 781 units/μg, which is calibrated against human BMP-2 WHO Standard (NIBSC code: 93/574).
• Molecular Mass: Predicted Molecular Mass: 13 (monomer) kDa
  SDS-PAGE: 15-16 kDa, reducing conditions
• N-terminal Sequence Analysis: Gln-Ala-Lys-His-Lys-Gln-Arg-Lys-Arg-Leu
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643