Active GMP Recombinant Human INHBA Protein

• Cat.No.: INHBA-114HG
• Product Overview: Recombinant Human INHBA, Gly311-Ser426, was produced in CHO cell under cGMP guidelines.

Specification

• Source: CHO cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
  The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626).
• Molecular Mass: Predicted Molecular Mass: 13 (monomer) kDa
  SDS-PAGE: 14 kDa, reducing conditions
  24 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A;inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476