Active Recombinant Bacterial Pyrimidine nucleoside phosphorylase, His tagged

• Cat.No.: PyNP-05B
• Product Overview: Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only. The pyrimidine nucleoside phosphorylase is of bacterial origin and thermostable up to 60 centigrade.

Specification

• Species: Bacterial
• Source: E.coli
• Tag: His
• Description: Pyrimidine nucleoside phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and pentose-1-phosphate. The enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.
• Form: Liquid, buffered
• Molecular Mass: 46.8 kDa
• Storage: 4 centigrade
• EC No.: 2.4.2.2
• CAS No.: 9055-35-0
• Catalyzed reaction(s): (1) uridine + phosphate = uracil + α-D-ribose 1-phosphate (2) 2'-deoxyuridine + phosphate = uracil + 2-deoxy-α-D-ribose 1-phosphate (3) thymidine + phosphate = thymine + 2-deoxy-α-D-ribose 1-phosphate
• Example reaction conditions: N/A Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
• Other characteristics: Thermostable up to 60 centigrade

Gene Information

• Official Symbol: PyNP
• Synonyms: PyNP; Py-NPase; PYNP; pynpase; pyrimidine ribonucleoside phosphorylase; pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase