Active Recombinant Bovine MMP1 protein, His-tagged

• Cat.No.: MMP1-4643B
• Product Overview: Active Recombinant Bovine MMP1 protein(P28053)(19~469 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Bovine
• Source: E.coli
• Tag: His
• Protein Length: 19-469 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: MMP1 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin and so on. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain. The activity of recombinant bovine MMP1 is measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rbMMP1 is diluted to 50 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37°C for 2 hours. The activated rbMMP-1 is diluted to 1 ug/mL in assay buffer. Loading into a black well plate 50 µL of 1 ug/mL rbMMP-1 and start the reaction by adding 50 µL of 20 µM substrate, with a substrate blank containing 50 µL assay buffer, 50 µL substrate, and no rbMMP-1. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant bovine MMP1 is > 150 pmol/min/µg.
• Molecular Mass: 55.1kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.