Active Recombinant Bovine TNF Protein

• Cat.No.: TNF-20B
• Product Overview: Recombinant Bovine TNF-alpha (78-234aa, 158aa) without tag was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity).
  
  Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.
• Source: E. coli
• Species: Bovine
• Form: Liquid
• Bio-activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15 ng/mL.
• Molecular Mass: 17.5 kDa
• Protein length: 78-234
• AA Sequence: MLRSSSQASSNKPVAHVVADINSPG QLRWWDSYANALMANGVKLEDNQLV VPADGLYLIYSQVLFRGQGCPSTPL FLTHTISRIAVSYQTKVNILSAIKS PCHRETPEWAEAKPWYEPIYQGGVF QLEKGDRLSAEINLPDYLDYAESGQ VYFGIIAL
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: TNF tumor necrosis factor [ Bos taurus (cattle) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNFa; TNF-a; TNF-alpha; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
• Gene ID: 280943
• mRNA Refseq: NM_173966
• Protein Refseq: NP_776391
• UniProt ID: Q06599