Active Recombinant Canine BMPR1A protein, hFc-tagged

• Cat.No.: BMPR1A-1043C
• Product Overview: Active Recombinant Canine BMPR1A protein(NP_001138622.1)(Gln24-Arg152) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: 24-152 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.5 - 2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The protein has a predicted MW of 40.94 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.