Active Recombinant Canine BMPR1A protein(Met1-Arg152), hFc-tagged

• Cat.No.: BMPR1A-1043C
• Product Overview: Recombinant Canine BMPR1A (NP_001138622.1) (Met1-Arg152) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Canine
• Tag: Fc
• Protein length: Met1-Arg152
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.5-2.5 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant Canine BMPR1A consists 370 amino acids and predicts a molecular mass of 41.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

11/06/2021

The enzyme activity of this protein reached the expected level and was able to play a role in the specific reaction.

10/20/2021

When experimenting with this protein, stable and repeatable results can be obtained.

03/08/2021

This product has strict quality control and complies with relevant industry standards.

Q&As

What disease might abnormal BMPR1A levels suggest?

Abnormal levels of BMPR1A may suggest skeletal dysplasia, tumors, etc., but its specificity and sensitivity need to be further studied.

Is there a mutation type of BMPR1A?

The types of mutations in BMPR1A include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

How are mutations in BMPR1A detected and analyzed?

Mutations in BMPR1A can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

How to study the regulatory mechanism of BMPR1A?

Studying the regulatory mechanism of BMPR1A requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

What are the applications of BMPR1A in medical treatment?

BMPR1A can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting BMPR1A, such as gene therapy or modulating its expression, are also being studied.

What diseases can BMPR1A deficiency or aberrant expression cause?

Deficiency or abnormal expression of BMPR1A may be related to some skeletal dysplasia diseases, tumors, etc.