Active Recombinant Canine CA9 Protein, His-tagged

• Cat.No.: CA9-1039C
• Product Overview: Recombinant canine CA9 (NP_001138646.1) (Met1-Leu410) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 1-410 a.a.
• Predicted N Terminal: Gln 38
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its esterase activity.The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The recombinant canine CA9 comprises 384 amino acids and has a predicted molecular mass of 42.1 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CA9 carbonic anhydrase IX [ Canis lupus familiaris ]
• Official Symbol: CA9
• Synonyms: carbonic anhydrase 9
• Gene ID: 611933
• mRNA Refseq: NM_001145174
• Protein Refseq: NP_001138646
• Chromosome Location: chromosome: 11
• Pathway: Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Reversible hydration of carbon dioxide, organism-specific biosystem