Active Recombinant Canine IL22 protein, His-tagged, Low Endotoxin

• Cat.No.: Il22-9516C
• Product Overview: Recombinant Canine IL22 protein(E2R2K7)(34-179 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 34-179 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Canine IL-22, His Tag at 5μg/ml(100μl/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 40.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.43 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.