Active Recombinant Canine MS4A1 protein, His-tagged

• Cat.No.: MS4A1-3049C
• Product Overview: Recombinant Canine MS4A1 protein(Met 1-Pro 297), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Pro 297
• Form: Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Canine CD20 Full Length Protein, His Tag at 5 μg/mL (100 μL/well) can bind Anti-Dog CD20 Antibody, Rabbit IgG with a linear range of 5-78.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.2 kDa. The protein migrates as 25 kDa and 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution. The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.