| Species : |
Canine |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
77-233 a.a. |
| Form : |
Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : |
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.01-0.08 ng/ml. |
| Molecular Mass : |
The recombinant canine TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band. |
| Proteinlength : |
Val77-Leu233 |
| Purity : |
> 96 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
