| Species : |
Canine |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
51-183 aa |
| Description : |
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart. |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Canine OX40 Ligand Protein, His Tag at 5 μg/mL (100 μL/well) can bind Canine OX40 Protein, Fc Tag with a linear range of 0.1-4 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 48.2 kDa. The protein migrates as >180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Endotoxin : |
Less than 0.1 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
