Active Recombinant Cotton Rat Tnf, Met-tagged

• Cat.No.: Tnf-391C
• Product Overview: Recombinant Cotton Rat Tnf (Accession # AAL18818) Leu1-Leu156, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Cotton Rat
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
• Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.03-0.12 ng/mL.
• Molecular Mass: Recombinant Cotton Rat Tnf, Met-tagged has a calculated MW of 17.5 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Protein length: Leu1-Leu156

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Tnf
• Synonyms: Tnf; tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
• Gene ID: 24835
• mRNA Refseq: NM_012675
• Protein Refseq: NP_036807
• UniProt ID: P16599
• Chromosome Location: 20p12