Source : |
HEK293 |
Species : |
COVID-19 |
Tag : |
His |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant. |
Bio-activity : |
Immobilized Human ACE2, Fc Tag at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (BA.2.3.20/Omicron) with a linear range of 0.1-2 ng/mL.Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2.3.20/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 with a linear range of 0.002-0.05 μg/mL.Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2.3.20/Omicron) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 138.2 kDa. The protein migrates as 165-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Val 16 - Pro 1213 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |