Active Recombinant COVID-19 Spike RBD Protein, His Tag (BA.2/Omicron), His-tagged
Cat.No. : | Spike-4653V |
Product Overview : | Recombinant COVID-19 Protein, His Tag (BA.2/Omicron)(Arg 319 - Lys 537), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) with a linear range of 4-31 ng/mL.Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.4-13 ng/mL.Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 1.844 μg/mL.Serial dilutions of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (MALS verified) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 2.038 μg/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Arg 319 - Lys 537 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
◆ Lysates | ||
Spike-001HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
Spike-001SCL | Recombinant SARS Spike cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket