Active Recombinant COVID-19 Spike S1 (D614G) protein, His-tagged
Cat.No. : | Spike-219V |
Product Overview : | Recombinant COVID-19 Spike S1 (D614G) protein (Val16-Arg685) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293). |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Form : | Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind 2019-nCoV S1(D614G)h, the EC50 of 2019-nCoV S1(D614G) is 250-650 ng/mL. 2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein with an affinity constant of 57.7 nM as determined by Octet RED System. |
Molecular Mass : | The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.41 kDa. |
Protein Length : | Val16-Arg685(D614G) |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Products Types
◆ Lysates | ||
Spike-001HCL | Recombinant HCoV-EMC/2012 Spike cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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