Active Recombinant Cynomolgus CD3E&CD3D protein(Primary Amine Labeling), hFc-tagged, Biotinylated

• Cat.No.: CD3E & CD3D-5234C
• Product Overview: Biotinylated Active Recombinant Cynomolgus CD3E&CD3D protein(Primary Amine Labeling)(Q95LI5.2 (CD3E) & Q95LI8 (CD3D))(Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D)) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 22-117 aa(CD3E) & 22-105 aa(CD3D)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Anti-CD3 Antibody, hFc Tag at 2μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa (CD3E) and 44-46 kDa (CD3D) based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.