| Species : |
Cynomolgus |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
Gln 22-Asp 117 (CD3E) & Gln 23-Thr 113 (CD3G) |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated Human BCMA, Fc,Avitag™, at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.5 ng/mL. |
| Molecular Mass : |
Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.0 kDa (CD3E) and 14.4 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 18-22 kDa due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
