Active Recombinant Cynomolgus CD3E protein, hFc-Avi-tagged, Biotinylated

• Cat.No.: CD3E-5633CB
• Product Overview: Biotinylated Recombinant Cynomolgus CD3E protein(Q95LI5.2)(Asp22-Thr48) is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 22-48 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Anti-CD3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 22-48, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.