Active Recombinant Cynomolgus DLL3 protein, His-tagged, Low Endotoxin

• Cat.No.: DLL3-1870C
• Product Overview: Recombinant Cynomolgus DLL3 protein(A0A2K5WSR1)(27-488 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 27-488 aa
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl, 200 mM L-arginine (pH 8.5). Normally 8% mannitol is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus DLL3, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49.21 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.