Active Recombinant Cynomolgus ERBB2, His-tagged
Cat.No. : | ERBB2-206C |
Product Overview : | A DNA sequence encoding the cynomolgus ERBB2 (Met1-Thr652) [Rhesus ERBB2 (XP_001090430.1,Asp121Asn,Leu122Pro); similar to Macaca fascicularis ERBB2 (EHH58073.1)] was expressed with a polyhistidine tag at the C-terminus. |
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- Gene Information
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Species : | Cynomolgus |
Source : | HEK293 |
Tag : | His |
Protein Length : | Met1-Thr652 |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | Immobilized Her2/ERBB2 Protein, Cynomolgus, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 6-40 ng/mL. |
Molecular Mass : | The recombinant cynomolgus ERBB2 comprises 641 amino acids and has a calculated molecular mass of 70.8 KDa. The apparent molecular mass of it is approximately 80-105 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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Not For Human Consumption!
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