Active Recombinant Cynomolgus/Rhesus macaque FCGRT & B2M protein, His-tagged

• Cat.No.: FCGRT & B2M-1533C
• Product Overview: Active Recombinant Cynomolgus/Rhesus macaque FCGRT & B2M protein(Q8SPV9(FCGRT) & Q8SPW0(B2M))(Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M)) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 24-297 aa(FCGRT) & 21-119 aa(B2M)
• Form: Lyophilized from 0.22 μm filtered solution in 20mM PB, 250mM NaC (pH 8.0).
• Bio-activity: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.5 kDa (FCGRT) and 11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.