Active Recombinant Human FCGRT&B2M protein, His tagged
Cat.No. : | FCGRT&B2M-3802H |
Product Overview : | Recombinant Human FCGRT (P55899) extracellular domain (Met 1-Ser 297), fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Human B2M (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M he |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | 1. Measured by its ability to bind human IgG1 in a functional ELISA. 2.Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 µM as determined in a BLI assay (ForteBio Octet Red384). 3.Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 µM as determined in a BLI assay (ForteBio Octet Red384). 4.Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 µM as determined in an SPR assay (Biacore T200). 5.Captured Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 µM as determined in an SPR assay (Biacore T200). 6.The purity of Human FCGRT & B2M Heterodimer Protein is more than 90% and the molecular weight of this protein is around 45-53 kDa verified by SEC-MALS. |
Molecular Mass : | The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Ser297&Met1-Met119 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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