Active Recombinant Rat FCGRT&B2M protein, His-Avi-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-1828R
• Product Overview: Biotinylated Active Recombinant Rat FCGRT&B2M protein(P13599(FCGRT) & P07151(B2M))(Ala23-Ser298 (FCGRT) & Ile21-Met119 (B2M)) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-298 aa(FCGRT) & 21-119 aa(B2M)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Rat FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 33.9 kDa (FCGRT) and 11.63 kDa (B2M). Due to glycosylation, the protein migrates to 45-60 kDa (FCGRT) and 12-14 kDa (B2M) based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Fcgrt Fc fragment of IgG, receptor, transporter, alpha [ Rattus norvegicus ]
• Official Symbol: FCGRT
• Gene ID: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; neonatal Fc receptor; Fc fragment immunoglobulin G receptor; Fc receptor, IgG, alpha chain transporter; igG Fc fragment receptor transporter alpha chain; FcRn;
• mRNA Refseq: http://www.ncbi.nlm.nih.gov/gene/29558
• Protein Refseq: http://www.ncbi.nlm.nih.gov/nuccore/NM_033351.1
• MIM: http://www.ncbi.nlm.nih.gov/protein/NP_203502.1
• Gene Name 2: B2m beta-2 microglobulin [ Rattus norvegicus ]
• Official Symbol 2: B2M
• Synonyms 2: B2M; beta-2 microglobulin; beta-2-microglobulin
• Gene ID 2: 24223
• mRNA Refseq 2: NM_012512
• Protein Refseq 2: NP_036644