Active Recombinant Cynomolgus GPC3 protein, lFc-tagged

Cat.No. : GPC3-1515C
Product Overview : Recombinant Cynomolgus GPC3 protein(Gln25-His559), fused with lFc tag, was expressed in HEK293.
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Source : HEK293
Species : Cynomolgus
Tag : lFc
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5.
Bio-activity : Immobilized Human FGF basic, premium grade at 1 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag with a linear range of 1-63 ng/mL.Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
Molecular Mass : This protein carries a llama IgG2b Fc tag at the C-terminus.
This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 50.5 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Gln25-His559
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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