Active Recombinant Cynomolgus GPC3 protein, His-tagged, Low Endotoxin

• Cat.No.: GPC3-1515C
• Product Overview: Recombinant Cynomolgus GPC3 protein(XP_005594665.1)(25-559 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 25-559 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus GPC3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 30-31 kDa, 38-42 kDa and 65-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.