Active Recombinant Cynomolgus IgE Protein, His-Avi-tagged, Biotinylated

• Cat.No.: IgE-909CB
• Product Overview: Biotinylated Recombinant Cynomolgus IgE Protein(G8F4W7)(Cys209-Lys429) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: 209-429 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IgE, His Avi Tag with the EC50 of 10.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Gene Information

• Official Symbol: IgE