Active Recombinant Cynomolgus IL2RB protein, His-tagged, Low Endotoxin

• Cat.No.: IL2RB-0281C
• Product Overview: Active Recombinant Cynomolgus IL2RB protein(Q38J85)(Ala27-Asp239) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 27-239 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.17 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.