Active Recombinant Cynomolgus LRRC32 & TGFB1 protein, His-tagged

• Cat.No.: LRRC32 & TGFB1-0324C
• Product Overview: Recombinant Cynomolgus LRRC32 & TGFB1 protein(Ala18-Asn627(LRRC32)&Leu30-Ser390(TGF-beta1)), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 18-627;30-390 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1-39 ng/mL.Immobilized Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Mouse IgG1:Purified anti-human LAP(TGF-β1) with a linear range of 0.5-8 ng/mL.
• Molecular Mass: The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature TGF-beta 1) due to glycosylation respectively.
• Proteinlength: Ala18-Asn627(LRRC32)&Leu30-Ser390(TGF-beta1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: LRRC32