Active Recombinant Mouse LRRC32 & TGFB1 protein, His-tagged

• Cat.No.: LRRC32 & TGFB1-0325M
• Product Overview: Recombinant Mouse LRRC32 & TGFB1 protein(Ile18-Asn628(LRRC32)&Leu30-Ser390(TGF-beta1)), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 18-628;30-390 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Purified anti-mouse LAP (TGF-β1) Antibody with a linear range of 0.2-1 ng/mL.
• Molecular Mass: The reducing (R) Heterotrimer protein migrates as 80 kDa (LRRC32), 40 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.
• Proteinlength: Ile18-Asn628(LRRC32)&Leu30-Ser390(TGF-beta1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lrrc32 leucine rich repeat containing 32 [ Mus musculus ]
• Official Symbol: LRRC32
• Synonyms: LRRC32; leucine rich repeat containing 32; glycoprotein A repetitions predominant; Garp; AI426318; EG434215; D11S833Eh; D7H11S833E
• Gene ID: 434215
• mRNA Refseq: NM_001113379
• Protein Refseq: NP_001106850