Active Recombinant Cynomolgus / Rhesus macaque FGL1 protein, mFc-tagged

• Cat.No.: FGL1-1450C
• Product Overview: Recombinant Cynomolgus / Rhesus macaque FGL1 protein(Leu23-Ile312), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: mFc
• Protein Length: 23-312 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Biotinylated Human LAG-3, Fc,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Cynomolgus / Rhesus macaque FGL1, Mouse IgG2a Fc Tag with a linear range of 0.019-0.625 μg/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the N-terminus.The protein has a calculated MW of 60.8 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu23-Ile312
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.