Active Recombinant Cynomolgus/Rhesus macaque TNFSF15 protein, His-tagged

• Cat.No.: TNFSF15-0744C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque TNFSF15 protein(Leu72-Leu251), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus/Rhesus macaque TL1A / TNFSF15, His Tag at 5 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag with a linear range of 0.2-10 ng/mL. Immobilized Human DcR3, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque TL1A / TNFSF15, His Tag with a linear range of 0.3-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 22.3 kDa. The protein migrates as 27-28 kDa and 29-31 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu72-Leu251
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.