Active Recombinant Cynomolgus SIRPA protein, Fc-tagged

• Cat.No.: SIRPA-0703C
• Product Overview: Recombinant Cynomolgus SIRPA protein(Glu31-Asn370), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 31-370 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus / Rhesus macaque CD47, His Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus SIRP alpha, Fc Tag with a linear range of 0.01-0.3 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 63.6 kDa. The protein migrates as 70-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu31-Asn370
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.