Active Recombinant Cynomolgus SOST Protein, His-tagged, Low Endotoxin

• Cat.No.: SOST-0724C
• Product Overview: Active Recombinant Cynomolgus SOST Protein(XP_005584428.2)(Gln69-Tyr258) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 69-258 aa
• Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Cynomolgus SOST, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 22.58 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.