Source : |
HEK293 |
Species : |
Cynomolgus |
Tag : |
His |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : |
Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-6, His,Avitag with a linear range of 0.005-0.156 μg/mL. Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag with a linear range of 0.01-0.313 μg/mL. Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.005-0.313 μg/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 23.4 kDa. The protein migrates as 31-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Gln24-Tyr213 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |