Active Recombinant Cynomolgus TFRC protein, His-tagged, Biotinylated

• Cat.No.: TFRC-0786C
• Product Overview: Biotinylated Recombinant Cynomolgus TFRC protein(Cys89-Phe760), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.3-10 ng/mL. Immobilized Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling with a linear range of 0.3-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 77.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Cys89-Phe760
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.