Active Recombinant Dog CYP2D15 Protein

• Cat.No.: CYP2D15-116D
• Product Overview: Recombinant dog CYP2D15 protein without tag were coexpressed in E. coli.

Specification

• Species: Dog
• Source: E.coli
• Description: High activity for the hydroxylation of bunitrolol and imipramine; low activity on debrisoquine.
• Bio-activity: Cytochrome c Reductase Activity: 330 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Bufuralol 1’-hydroxylase
  Linear with time up to: 8 min(Determined at 3 µM substrate, using a P450 concentration of 5 pmol/ml)
  Linear with P450 up to: 15 pmol/ml(Determined at 3 µM substrate, using an incubation time of 5 min)
  Vmax: 5.4 pmol/min/pmol CYP
  Km: 6.2 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 5.2 nmol/ml
  Protein Concentration: 21.2 mg/ml
  Specific P450 Content: 245 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2D15 cytochrome P450 2D [ Canis lupus familiaris (dog) ]
• Official Symbol: CYP2D15
• Synonyms: CYP2D15; cytochrome P450 2D; CYP2D; CYPIID15; cytochrome P450 2D15; Cytochrome-P450-2D15; cytochrome P450 DUT2; cytochrome P450, family 2, subfamily D, polypeptide 15; monooxygenase
• Gene ID: 415120
• mRNA Refseq: NM_001003333
• Protein Refseq: NP_001003333
• UniProt ID: Q29473