Active Recombinant E.coli G6PD Protein (1-491aa)

• Cat.No.: G6PD-01E
• Product Overview: Recombinant E. coli G6PD protein (1-491aa) was expressed in E. coli and purified by conventional chromatography techniques.

Specification

• Description: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.
• Source: E. coli
• Species: E. coli
• Tag: Tag Free
• Form: Liquid
• Bio-activity: Specific activity is > 50 unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 centigrade.
• Molecular Mass: 55.7 kDa (491aa) confirmed by MALDI-TOF
• Protein Length: 1-491aa
• AA Sequence: MAVTQTAQACDLVIFGAKGDLARRK LLPSLYQLEKAGQLNPDTRIIGVGR ADWDKAAYTKVVREALETFMKETID EGLWDTLSARLDFCNLDVNDTAAFS RLGAMLDQKNRITINYFAMPPSTFG AICKGLGEAKLNAKPARVVMEKPLG TSLATSQEINDQVGEYFEECQVYRI DHYLGKETVLNLLALRFANSLFVNN WDNRTIDHVEITVAEEVGIEGRWGY FDKAGQMRDMIQNHLLQILCMIAMS PPSDLSADSIRDEKVKVLKSLRRID RSNVREKTVRGQYTAGFAQGKKVPG YLEEEGANKSSNTETFVAIRVDIDN WRWAGVPFYLRTGKRLPTKCSEVVV YFKTPELNLFKESWQDLPQNKLTIR LQPDEGVDIQVLNKVPGLDHKHNLQ ITKLDLSYSETFNQTHLADAYERLL LETMRGIQALFVRRDEVEEAWKWVD SITEAWAMDNDAPKPYQAGTWGPVA SVAMITRDGRSWNEFE
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT

Gene Information

• Gene Name: zwf NADP(+)-dependent glucose-6-phosphate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: zwf
• Synonyms: zwf; NADP(+)-dependent glucose-6-phosphate dehydrogenase; ECK1853; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.
• Gene ID: 946370
• Protein Refseq: NP_416366
• UniProt ID: P0AC53