| Species : |
Elizabethkingia miricola |
| Source : |
E. coli |
| Tag : |
Non |
| Description : |
Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins.
Endo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. |
| Form : |
Sterile-filtered solution |
| EC : |
3.2.1.96 |
| Specificity : |
Cleaves all asparagine-linked high mannose and some hybrid oligosaccharides.
Ludger Endo F1 cleaves Asparagine-linked high mannose or hybrid oligosaccharides. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. |
| Contents : |
60 μL aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5
5× Reaction Buffer-250 mM sodium phosphate, pH 5.5 |
| Bio-activity : |
Activity: ≥ 17 U/mL
Specific Activity: ≥ 16 U/mg
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37 centigrade, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster). |
| Molecular Mass : |
32 kDa |
| Suggested usage : |
1. Add up to 200 μg of glycoprotein to an Eppendorf tube.
2. Adjust to 38 μL final volume with de-ionized water. Add 10 μL 5× Reaction Buffer 5.
3. Add 2.0 μL of Endo F1 to the reaction. Incubate 1 hour or more at 37 centigrade.
4. Monitor cleavage by SDS-PAGE. |
| Purity : |
Ludger Endo F1 is tested for contaminating protease as follows: 10 μg of denatured BSA is incubated at 37 centigrade for 24 hours with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases. |
| Stability : |
Several days exposure to ambient temperatures will not reduce activity. Stable at least 12 months when stored properly. |
| Storage : |
Store enzyme at 4 centigrade. Do not freeze. |
| Storage Buffer : |
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, pH 7.5 |
