Active Recombinant Full Length Human CD33 Protein, C-Flag-tagged

• Cat.No.: CD33-139HFL
• Product Overview: Recombinant Full Length Human CD33 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Association in cell culture
• Molecular Mass: 38 kDa
• AA Sequence: MPLLLLLPLLWAGALAMDPNFWLQV QESVTVQEGLCVLVPCTFFHPIPYY DKNSPVHGYWFREGAIISGD SPVATNKLDQEVQEETQGRFRLLGD PSRNNCSLSIVDARRRDNGSYFFRM ERGSTKYSYKSPQLSVHVTD LTHRPKILIPGTLEPGHSKNLTCSV SWACEQGTPPIFSWLSAAPTSLGPR TTHSSVLIITPRPQDHGTNL TCQVKFAGAGVTTERTIQLNVTYVP QNPTTGIFPGDGSGKQETRAGVVHG AIGGAGVTALLALCLCLIFF IVKTHRRKAARTAVGRNDTHPTTGS ASPKHQKKSKLHGPTETSSCSGAAP TVEMDEELHYASLNFHGMNP
  SKDTSTEYSEVRTQTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens (human) ]
• Official Symbol: CD33
• Synonyms: p67; SIGLEC3; SIGLEC-3
• Gene ID: 945
• mRNA Refseq: NM_001772.4
• Protein Refseq: NP_001763.3
• MIM: 159590
• UniProt ID: P20138